Description
Aviva Systems Biology ALB ELISA Kit (Pigeon) (OKCA00370) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for pigeon ALB has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for pigeon ALB is added, incubated and then followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP producing a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample pigeon ALB captured in well